• [Archive]
• Journal of Pharmacopuncture
• pISSN : 2093-6966
• eISSN : 2234-6856
• DB Construction : 59 Issues, 667 Articles
Year
Issues
Articles
1 Title
Keywords been venom; anti-cancer effect; molecular biology
Author(s) Park, Chan-Yol; Seo, Jung-Chul; Choi, Do-Young; Ahn, Byoung-Choul
 Abstract To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, $\small{[^3H]}$thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease activity assay. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis-related genes was performed. The obtained results are summarized as follows: 1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2. Significant induction of apoptosis was identified using tryphan blue assay, $\small{[^3H]}$thymidine release assay, and flow cytomet1 ric analysis of sub $\small{G_1}$ fraction. 3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-$\small{X_L}$ were down-regulated whereas Bax was up-regulated by bee venom treatment.
2 Title
Keywords
Author(s) 대한약침학회
 Abstract
3 Title
Keywords apitoxin; pain; serotonin; TPH; phopholipase; prostaglandin; RT-PCR
Author(s) Cho, Kwang-Ho; Lee, Jae-Dong; Park, Dong-Suk; Ahn, Byoung-Choul
 Abstract The purpose of this study is to prove the analgesic effects of apitoxin and its mechanism via jaw-opening reflex(JOR) and measuring expression of mRNA in Phospholipase and Tryptophan hydroxylase(TPH) using RT-PCR. The experiments were carried out on Sprague-Dawley rats(300-400g) and mastocytoma(P-185 HTR) for JOR and RT-PCR, respectively. Rats anesthetized with thiopental sodium (80mg/kg) were used in the Tooth Pulp stimulation induced JOR. The amplitude of a digastric electromyogram (dEMG) was recorded during the stimulation at an intensity of 1.5 times the threshold for JOR. Apitoxin used in this experiment was diluted with normal saline by 1:1000. Apitoxin was injected intravenously into the test group while normal saline to the control group. However, it was injected directly into the cell of mastocytoma. We referred to base sequence registered in Genbank in designing primers for RT-PCR. The results were as follows; (1)Compared with control group, analgesic effect started to show right after Sprague-Dawely rats were treated with apitoxin($\small{71.50{\pm}8.08}$) and lasted for 50 minutes. (2)As a result of the experiment of RT-PCR, we witnessed significant changes in the degree of expression of phospholipase or rate-limiting enzyme of biosynthesis of prostaglandins with $\small{10{\mu}g/ml}$ apitoxin.($\small{31.74{\pm}18.98%}$, P<0.05) (3)As a result of the experiment of RT-PCR, we witnessed significant changes in the degree of expression of TPH or rate-limiting enzyme in biosynthesis of serotonin with $\small{10{\mu}g/ml}$ apitoxin.($\small{131.37{\pm}16.87%}$, P<0.05). These results suggest that $\small{10{\mu}g/ml}$ apitoxin have the most analgesic effects. This study showed that apitoxin has analgesic effects and held good for 50 minutes. The injection of apitoxin has brought out changes in the degree of expression of phospholipase and TPH. These results strongly suggest that analgesic mechanism by apitoxin is closely related to prostaglandins and serotonin.
4 Title
Keywords Carthami oil; Acuapuncture; Safety; Irritation and Toxicity
Author(s) Lim, Sa-Bi-Na; Kang, Dong-Chul
 Abstract Carthami tinctorii Fructus is known for its good effect on diseases such as fracture, menorrhalgia, menoschesis, puerperium aneilema and so on. The study of irritation and toxicity of Carthami oil aquapuncture solution applied topically to the skin and the eye mucous membrane were carried out to prove the safety of Carthami oil aquapuncture solution in clinical use. Animal for the research was the rabbit and the solution for the test was made from Carthami semen. 6 animals were used for the skin test and 9 animals were used for the eye mucous membrane test. In results, both tests proved that Carthami oil aquapuncture solution makes no irritable reaction on skin and eye mucous membrane of rabbit. We consider that this result is helpful for saying about the safety of Carthami oil aquapuncture solution in clinical use.
5 Title
Keywords Bee venom; Acupuncture; pain; Fos protein; Catecholamine; Immunohistochemistry
Author(s) Kim, Hye-Nam; Nam, Sang-Soo; Lee, Yun-Hoo; Choi, Yong-Tae
 Abstract This study was designed to evaluate the effect of the bee venom(BV) aqua-acupuncture on the neuronal activities of catecholaminergic(tyrosine hydroxylase : TH, dopamine $\small{{\beta}}$ hydroxylase : D$\small{{\beta}}$H) system in the brainstem. After the BV aqua-acupuncture was applied on Chok-Samni(ST36) and the gluteal part(Blank locus) in rats. Also, the number of colocalization between catecholamine containing neurons and Fos immunoreactive neurons were analyzed by using the double immunohistochemical technique. The results of the experiments were summarized as follows : 1. In DR and LC, Chok-Samni group and the Blank locus group showed more significant increase in the number of colocalization between TH containing neurons and Fos immunoreactive neurons than the control group. Furthermore, Chok-Samni group showed more significant increase than the Blank locus group. Also, in Arc, Chok-Samni group showed more significant increase than the Blank locus group and the control group. 2. In LC, Chok-Samni group showed more significant increase in the number of colocalization between D$\small{{\beta}}$H containing neurons and Fos immunoreactive neurons than the Blank locus group and the control group. Also, in A5, Chok-Samni group and the Blank locus group showed more significant increase than the control group. Chok-Samni group showed more significant increase than the Blank locus group. However, there was no significant change in A7. Consequently, the BV aqua-acupuncture increased more potent the number of Fos immunoreactive neurons and the activity of catecholaminergic neurons. Furthermore, the BV aqua-acupuncture was more effective on Chok-Samni than Blank locus group. These results indicate that the BV aqua-acupuncture is very effective therapy to control pain. The therapeutic effect of BV aqua-acupunture may associated with the endogenous modulatory system such as catecholamine. Those data from the study can be applied to establish the effective treatment of the BV for pain control in the clinical field.
6 Title
Keywords
Author(s) Yun, Jeong-Hun; Yook, Tea-Han; Song, Beom-Yong
 Abstract This report was done to observe the effect of Hominis placenta herbal acupuncture on Bell's palsy. The study group comprised 16 patients who arrived at Woo-suk university oriental hospital from January, 1999 till January, 2000 for Bell's palsy. All patients were divided into two group. One was herbal acupunture group, and the other was control group. Acupunture group was done herbal acupuncture therapy on the facial acupuncture points. Followings are achievement and a term of each group. In herbal acupuncture group, 100% motor recovery was 7 case, 75% was 1 case, and 25% motor recovery term was $\small{7.38{\pm}5.21}$ days, 50% was $\small{11.00{\pm}6.16}$ days, 75% was $\small{15.13{\pm}9.55}$ days, 100% was $\small{23.14{\pm}7.97}$ days. In control group, 100% motor recovery was 4 case, 75% was 2 case, 25% below was 2 case and 25% motor recovery term was $\small{11.17{\pm}4.96}$days, 50% was $\small{18.17{\pm}6.82}$ days, 75% was $\small{29.50{\pm}6.95}$ days, 100% was $\small{44.00{\pm}11.49}$ days. The above results indicate that Hominis placenta herbal acupuncture is a useful effect on Bell's palsy. thus, continuous herbal acupunture study will be needed for more clinical application on Bell' palsy.
7 Title
Keywords Bee Venom aqua-acupunture;$\small{Chungwan(CV_{12})}$ ; writhing reflex
Author(s) Jeong, Sun-Hee; Koh, Hyung-Kyun; Park, Dong-Suk
 Abstract Introduction : In spite of the use of Bee Venom aqua-acupuncture in the clinics, the scientific evaluation on effects is not enough. Bee Venom aqua-acupuncture is used according to the stimulation of acupuncture point and the chemical effects of Bee Venom. The aims of this study is to investigate the analgegic effects of the Bee Venom aqua-acupuncture, through the change of writhing reflex Materials and Methods : Pain animal model was used acetic acid method. The changes of writhing reflex of the mice which were derived pain by injecting acetic acid into the abdomen, after stimulating Bee Venom aqua-acupuncture on Chungwan($\small{CV_{12}}$) and non acupuncture point on the backside were measured. Results : 1. It showed that the writhing reflex were appeared on the groups which injected aceticacid only, and saline-acetic acid group(sample I), but not on the group bee venom-saline group(sample II). 2. The change of writhing reflex by Chungwan($\small{CV_{12}}$) Bee Venom aqua-acupuncture showed significant decrease in the order of Chungwan($\small{CV_{12}}$) Bee Venom aqua-acupuncture group III($\small{2.5{\times}10^{-3}}$g/kg), II($\small{2.5{\times}10^{-4}}$g/kg), and I($\small{2.5{\times}10^{-5}}$g/kg), compared with control group. There were significant decrease of number of writhing reflex in $\small{5{\sim}10,\;10{\sim}15\;and\;15{\sim}20}$ minutes intervals of Chung wan($\small{CV_{12}}$) Bee Venom aqua-acupuncture group I, and in $\small{0{\sim}5,\;5{\sim}10,\;10{\sim}15\;and\;15{\sim}20}$ minutes intervals of II and III, compared with control group. 3. The change of writhing reflex by non acupuncture point Bee Venom aqua-acupuncture showed significant decrease in the $\small{0{\sim}5\;and\;5{\sim}10}$ minutes intervals and the total number of writhing reflex in $\small{2.5{\times}10^{-4}}$g/kg group, compared with control group 4. The effects of writhing reflex of Chungwan($\small{CV_{12}}$) Bee Venom aqua-acupuncture group showed significant decrease, compared with non acupuncture point Bee Venom aqua-acupuncture group. Conclusion : This study shows that the Bee Venom aqua-acupuncture on Chungwan($\small{CV_{12}}$) decreases the numbers of writhing reflex. As the analgegic effects of Bee Venom aqua-acupuncture is recognized. Bee Venom aqua-acupuncture treatment is expected for pain modulation. In order to use it in many ways, more researches are needed for the dose and stability of Bee Venom aqua-acupuncture.
8 Title
Keywords Bee Venom therapy; progressive muscle atrophy
Author(s) Kim, Young-Ho; Yook, Tae-Han; Song, Beom-Yong; Lee, Dong-Ho;
 Abstract The authors reports in order to study the effect of Bee Venom therapy of progressive muscle atrophy. The authors investigated 1 patient who is treated at Woosuk University Oriental Medical Hospital. The patient diagnosed by MRI EMG Hematology Muscle biopsy as progressive muscle atrophy is administered by Bee Venom therapy for 4 months. Bee Venom therapy is operated by 2 times per a week(every 3 days, 0.1cc per one operation, 0.05cc per one acupuncture point). The authors checked changes of this patient's chief symptoms by comparing before and after Bee Venom therapy is operated at 30 times. After Bee Venom therapy, the patient increased motor power & ROM, decreased general cooling sense & swallowing disorder. As above, the authors conclude that better results can be obtained Oriental Medical Treatment with Bee Venom therapy in progressive muscle atrophy
9 Title
Keywords bee venom (BV) acupuncture; Chok-samni (ST36); Fos-like immunoreactive (Fos-LI) neuron
Author(s) Yim, Yun-Kyoung; Kang, Sung-Keel; Choi, Do-Young
 Abstract This study was designed to evaluate the analgesic effect of bee venom (BV) Acupuncture into different treatment points, Chok-samni (ST36) and blank loci of the gluteal muscle and back. We investigated neuronal activity in the spinal cord using the Fos immunohistochemical technique according to the pretreatment with different concentrations of BV, thirty minutes before the formalin injection. The results were summarized as follows: 1. The number of Fos-like immunoreactive (Fos-LI) neurons in L2 segment of the saline-formalin treated group was significantly increased in NECK and VENT of the spinal cord as compared with that of the room control group. However, there was no significant change in the number of the Fos-LI neurons in L2 segment of the BV-formalin treated group as compared with that of the room control group. 2. The number of Fos-LI neurons in L3-5 segment of the saline-formalin group was significantly increased in all the regions of 142 the spinal cord as compared with that of the room control group. However, the Fos-LI neurons in L3-5 segment of the BV-formalin treated group was dramatically decreased in all the regions of the spinal cord as compared with that of the saline-formalin group. Therefore, these results indicated that the BV acupuncture suppressed the nociceptive neuronal activities in L3-5 of the spinal cord induced by formalin injection. 3. There was a strong positive correlation between the formalin-induced pain behavior and the number of the Fos-LI neurons in L3-5 segment.
10 Title
Keywords
Author(s) Yun, Hyoung-Seok; Kim, Young-Suk; Lee, Jae-Dong
 Abstract In order to study Bee venom and Pain, We searched Journals and Internet. The results were as follows: 1. The domestic papers were total 13. 4 papers were published at The journal of korean acupuncture & moxibustion society, 3 papers were published at The journal of korean oriental medical society, Each The journal of KyoungHee University Oriental Medicine and The journal of korean sports oriental medical society published 1 papers and Unpublished desertations were 3. The clinical studies were 4 and the experimental studies were 9. 2. The domestic clinical studies reported that Bee venom Herbal Acupuncture therapy was effective on HIVD, Subacute arthritis of Knee Joint and Sequale of sprain. In the domestic experimental studies, 5 were related to analgesic effect of Bee vnom and 4 were related to mechanism of analgesia. 3. The journals searched by PubMed were total 18. 5 papers were published at Pain, Each 2 papers were published at Neurosci Lett. and Br J Pharmacol, and Each Eur J Pain, J Rheumatol, Brain Res, Neuroscience, Nature and Toxicon et al published 1 paper. 4. In the journals searched by PubMed, Only the experimental studies were existed. 8 papers used Bee Venom as pain induction substance and 1 paper was related to analgesic effects of Bee venom. 5. 15 webpage were searched by internet related to Bee Venom and pain. 11 were the introduction related to arthritis, 1 was the advertisement, 1 was the patient's experience, 1 was the case report on RA, 1 was review article.
11 Title
Keywords Cervi Pantotricuhum Cornu; Herb-acupuncture; osteoporosis; ovariectomy;$\small{Shinsu(BL_{23})}$;$\small{Taejo(BL_{11})}$
Author(s) Han, Sang-Won; Lee, Yun-Ho; Kim, Chang-Hwan
 Abstract In order to investigate the effect of the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu($\small{BL_{23}}$) and Taejo($\small{BL_{11}}$) loci on the estrogen-deficiency osteoporosis induced by ovariectomy in rats, serum osteocalcin estradiol progesterone follicular stimulating hormone(FSH) calcium(Ca) and phosphorous(P) levels were monitored. The results were as follows : 1. Serum osteocalcin levels were significantly (p<0.01, p<0.05) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 2. Serum estradiol levels were increased In the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group, but Taejo group did not show significant value. 3. Serum progesterone levels were significantly(p<0.01) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 4. Serum FSH levels were significantly(p<0.01, p<0.05) decreased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 5. Serum Ca levels were significantly(p<0.01) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu group compared with control group, but Taejo group did not show significant value. 6. Serum P levels were significantly(p<0.01, p<0.05) decreased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. According to these results, the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo loci have favorable effect on treatment and prevention of osteoporosis induced by ovariectomy in rats