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  • [Archive]
  • Journal of Pharmacopuncture
  • pISSN : 2093-6966
  • eISSN : 2234-6856
  • DB Construction : 59 Issues, 667 Articles
Year
Issues
Articles
1 Title
Keywords Venom; Sweet Bee Venom; preadipocyte; adipocytes; lipolysis
Author(s) Kim, Min-Ki; Lee, Si-Hyeong; Shin, Jo-Young; Kim, Kang-San; Cho, Nam-Guen; Rhim, Tae-Jin; Kwon, Ki-Rok
  Abstract Objectives The purpose of this study was to investigate the effects of Bee Venom and Sweet Bee Venom to the primary cultured preadipocyte, adipocytes, and localized fat tissue. Methods Decreased preadipocyte proliferation and decreased lipogenesis are mechanisms to reduce obesity. So, preadipocytes and adipocytes were performed on cell cultures using Sprague-Dawley Rats and treated with 0.01-1mg/ml Bee Venom and Sweet Bee Venom. And porcine skin including fat tissue after treated Bee Venom and Sweet Bee Venom according to the dosage dependent variation are investigated the histologic changes after injection of these Pharmacopuncture. Result Following results were obtained from the preadipocyte proliferation and lipolysis of adipocyte and histologic investigation of fat tissue. 1. Bee Venom and Sweet Bee Venom showed the effect of decreased preadipocyte proliferation depend on concentration. 2. Bee Venom and Sweet Bee Venom showed the effect of decreased the activity of glycerol-3-phosphate dehydrogenase(GPDH) significantly. 3. Bee Venom was not showed the effect of lipolysis, but Sweet Bee Venom was increased in low dosage and decreased in high dosage. 4. Investigated the histologic changes in porcine fat tissue after treated Bee Venom and Sweet Bee Venom, we knew that these Pharmacopuncture was activated nonspecific lysis of cell membranes depend on concentration. Conclusion These results suggest that Bee Venom and Sweet Bee Venom efficiently induces decreased proliferation of preadipocyte and lipolysis in adipose tissue.
2 Title
Keywords RAW 264.7; TNF-a; IL-1b; IL-6; NOS-II; nitric oxide (NO); Anti-inflammation
Author(s) Yoon, Mi-Young; Yoo, Hwa-Seung
  Abstract Aim This study was done to investigate whether SPP has inhibitory effects on the activation of RAW 264.7 cells. Method In tumor necrosis factor-a (TNF-a)/ interleukin-1b (IL-1b) and IL-6, the mRNA expression of molecular indicators related to inflammatory changes of the Reumatoid Arthritis (RA) were examined using quantitative real-time PCR. Results The treatment of SPP significantly suppressed the expression of proinflammatory cytokines and chemokines such as TNF-a, IL-1b, IL-6 compared with the control. The expression of NOS-II was considerably reduced, which was accompanied by a reduction in the production of nitric oxide (NO). It also reduced the expression of in serum of Balb/c mice compared with control group. Conclusion SPP is an effective herbal material for suppressing the inflammation related cytokines of RAW 264.7 cells.
3 Title
Keywords Cultivated wild ginseng; preadipocytes; adipocytes; obesity
Author(s) Kim, Byoung-Woo; Kwon, Ki-Rok
  Abstract Objectives The purpose of this study is to investigate the effects of cultivated wild ginseng extract on primary cultured preadipocyte and adipocytes. Methods Diminish preadipocyte proliferation does primary role to reduce obesity. So, preadipocytes and adipocytes were performed on cell cultures with using Sprague-Dawley rats and treated with 0.01-1mg/ml cultivated wild ginseng extract. Result At all concentrations, cultivated wild ginseng extract wasn't show the suppress proliferation of preadipocytes significantly and failed to show effects on decomposition of adipocytes except high dosage. Conclusion Based on these findings, cultivated wild ginseng is not a suitable choice for the treatment of localized obesity.
4 Title
Keywords Ganoderma lucidum herbal acupuncture(GHA); Ganoderma lucidum extract(GE); allergy; RBL-2H3; Raw264.7
Author(s) Kang, Kyung-Hwa; Youn, Hyoun-Min
  Abstract Objectives We studied on anti-allergic effects of Ganoderma lucidum herbal acupuncture(GHA) and Ganoderma lucidum extract(GE). Methods In vivo, Animals were herbal-acupunctured GHA at both B13s three times for 5 days. Then, we investigated compound 48/80-induced active systemic anaphylatic shock using ICR mice and anti-DNP IgE-induced passive cutaneous anaphylaxis using Sprague Dawley rat. In vitro, we measured cell viability, b-hexosaminidase release, IL-4 and TNF-a from RBL-2H3 cells, and nitric oxide from Raw264.7 cell after treatment of GE of various concentrations. Results In vivo, GHA pretreatments at both B13s inhibited compound 48/80-induced active systemic anaphylatic shock. Passive cutaneous anaphylaxis were inhibited by GHA10 and OP. In vitro, GE treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF-a and nitric oxide. Conclusions These results suggest that GHA and GE may be beneficial in the inhibition of allergic inflammatory response.
5 Title
Keywords Staphylococcus aureus; keratitis; Coptidis rhizoma extract; MIC; Inhibition zone
Author(s) Seo, Hyeong-Sik
  Abstract Objective This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus aureus that induce keratitis. Methods Minimal inhibitory concentration(MIC) was measured by dropping to  according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples() in 100% density. 3. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples () in 100% density. Anti-bacterial potency of 80g Coptidis rhizoma extract decreased compared with 40g. Anti-bacterial potency of 160g Coptidis rhizoma extract decreased compared with 40g in . Conclusions Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, antibacterial potency compare with quantity and density must be studied.
6 Title
Keywords Puerariae Radix; antioxidative effects; free radical
Author(s) Eun, Young-Joon; Song, Yun-Kyung; Lim, Hyung-Ho; Kwon, Ki-Rok; Rhim, Tae-Jin
  Abstract Objective The objective of this study was to investigate the antioxidative effects of Puerariae Radix extract. Method Total antioxidant capacity (TAC), Total antioxidant response (TAR), Total phenolic content, Reactive oxygen species (ROS), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activities, lipid peroxidation were examined. Result Total antioxidant status was examined by total antioxidant capacity(TAC) and total antioxidant response(TAR) against potent free radical reactions. TAC and TAR of Puerariae Radix extract at the concentration of 5 mg/ml were 2.02 and 1.50 mM Trolox equivalents, respectively. Total phenolic content of Puerariae Radix extract at the concentration of 5 mg/ml was 2.29 mM gallic acid equivalent. Concentration of Puerariae Radix extract at which DPPH radical scavenging activity was inhibited by 50% was 5.91 mg/ml as compared to 100% by pyrogallol solution as a reference. The inhibitory effect of the extract on lipid peroxidation was examined using rat liver mitochondria induced by FeSO4/ascorbic acid. Puerariae Radix extract at the concentration of 1 mg/ml slightly but significantly decreased TBARS concentration. The extract further prevented lipid peroxidation in a dose-dependent manner. The effect of Puerariae Radix extract on reactive oxygen species (ROS) generation was examined using cell-free system induced by hydrogen peroxide/FeSO4. Addition of 1 mg/ml of Puerariae Radix extract significantly reduced dichloroflurescein (DCF) fluorescence. The extract caused concentration-dependent attenuation of the increase in DCF fluorescence, indicating that the extract significantly prevented ROS generation in vitro. Thus antioxidant effects of Puerariae Radix extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation, followed by inhibition of lipid peroxidation. Conclusion As a result, Puerariae Radix seems to have antioxitative effect and antioxidant compount.
7 Title
Keywords Sedang-Hwan; modified Sedang-Hwan; Streptozotocin; Rats; Diabetes Mellitus
Author(s) Lee, Chang-Geun; Soh, Kyeong-Sun; Jeong, Chan-Gil
  Abstract Objectives We performed this study in order to investigate the effects of Sedang-Hwan(世糖丸) and modified Sedang-Hwan(世糖丸變方)on the diabetes mellitus. Methods We injected a vein with 65mg/kg of streptozotocin(STZ) on the rats. And then administered Sedang-Hwan(Sample 1 group); 18.7mg/kg/day, modified Sedang-Hwan; 16.5mg/kg/day(Sample 2 group) to Sample groups and observed the body weight, glucose and insulin levels. Results 1. The Sample 1, 2 groups showed a high suppressive effect of body weight loss compared to Control group. 2. The Sample 1, 2 groups’ glucose level showed a effective in lowering level compared to Control group. 3. The sample 1, 2 groups showed a higher insulin level than Control group. Conclusions Conclusively, modified Sedang-hwan was recognized to have decrease effect of serum glucose of the diabetic rats induced by streptozotocin. It is also required to study on the further detailed mechanism of decrease effect of serum glucose by modified Sedang-hwan.
8 Title
Keywords Staphylococcus species; S. aureus; S. epidermidis; Coptidis rhizoma extract; eye disease; MIC; Inhibition zone
Author(s) Seo, Hyeong-Sik
  Abstract Objectives This experimental study was performed to investigate the continuous anti-bacterial potency of Coptidis rhizoma extract on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods Minimal inhibitory concentration(MIC) was measured by dropping to diluted Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(). Results 1. Anti-bacterial potency of Coptidis rhizoma extract on S. aureus was appeared in 100%, 10% and was the same as anti-bacterial potency of 2 days and 6 days. Anti-bacterial potency with change of volume(100%) was increased in propotion to increase volume on all samples. Anti-bacterial potency with change of volume(10%) was increased in propotion to increase volume on all samples except . Anti-bacterial potency of Coptidis rhizoma extract on S. aureus was appeared continuous. 2. Anti-bacterial potency of Coptidis rhizoma extract on S. epidermidis was appeared in 100%, 10% and was the same as anti-bacterial potency of 2 days and 6 days. Anti-bacterial potency with change of volume(100%) was increased in propotion to increase volume on all samples. Anti-bacterial potency with change of volume(10%) was appeared in . Anti-bacterial potency of Coptidis rhizoma extract on S. epidermidis was appeared continuous. Conclusions Anti-bacterial potency of Coptidis rhizoma extract on cultivation of S. aureus & S. epidermidis was showed continuous.
9 Title
Keywords Sweet Bee Venom; Whiplash; Traffic Accident; Herbal Acupuncture
Author(s) Song, Beom-Yong
  Abstract Objectives The aim of this study is to investigate the effect of Sweet Bee Venom herbal acupuncture for patients with acute whiplash injury by Traffic Accident. Methods This clinical study was carried out 25 cases of acute whiplash injury patients which had been treatment in Woosuk oriental hospital from March, 2007 to September, 2007. Sweet bee venom herbal acupuncture(N=15) and normal saline(N=10) injected on the acupoints that were cervical area. I checked the VAS for the pain and ROM(range of motion) of the cervical. these were checked 3 times. one was before treatments, another was after 3 times treatments with sweet bee venom herbal acupuncture and normal saline injection, and the other was after 5 times treatments with sweet bee venom herbal acupuncture and normal saline injection. Results VAS score was significantly improved after 5 times treatments with the sweet bee venom herbal acupuncture compared to normal saline I.M. on the acupoints that was cervical area. There were significant changes in the sweet bee venom herbal acupuncture group with VAS and ROM check. Conclusions This study suggests that sweet bee venom herbal acupuncture can improve symptoms in patients with acute whiplash injury by traffic accident.
10 Title
Keywords Bee Venom; Sweet Bee Venom; Low back pain; Radiating pain; Visual Analog Scale(VAS); Straight Leg Raising Test(S.L.R.T)
Author(s) Lee, Tae-Ho; Hwang, Hee-Sang; Chang, So-Young; Cha, Jung-Ho; Jung, Ki-Hoon; Roh, Jeong-Du; Lee , Eun-Young
  Abstract Objective The aim of this study is to investigate if Sweet Bee Venom therapy has the equal effect in comparison with Bee Venom Therapy on Low back pain with Radiation pain. Methods Clinical studies were done 24 patients who were treated low back pain with radiation pain to Dept. of Acupuncture & Moxibusition, of Oriental Medicine Se-Myung University from April 1, 2007 to September 30, 2007. Subjects were randomly divided into two groups ; Bee Venom treated group(Group A, n=10), Sweet Bee Venom treatred group(Group B, n=14). In Bee Venom treated group(Group A), we treated patients with dry needle acupuncture and Bee Venom therapy. In Sweet Bee Venom treatred group(Group B), we treated patients with dry needle acupuncture and Sweet Bee Venom therapy. All process of treatment were performed by double blinding method. To estimate the efficacy of controlling pain. we checked Visual Analog Scale(VAS). For evaluating functional change of patients, Straight Leg Raising Test(S.L.R.T) was measured. Results 1. In controlling pain, Sweet Bee Venom treatred group(Group B) had similar ability in comparison with Bee Venom treated group(Group A). 2. In promoting function, Sweet Bee Venom treatred group(Group B) had similar ability in comparison with Bee Venom treated group(Group A). Conclusions It may be equal effects as compared with using Bee Venom to treat low back pain with radiation pain using Sweet Bee Venom. We can try to treat other disease known to have effect with Bee Venom.
11 Title
Keywords Oriental Medicine therapy; Oriental and Western Medicine combination therapy; traffic accident
Author(s) Lee, Kyoung-Hee; Kim, Jung-Eun; Youn, Hyoun-Min; Ko, Woo-Shin; Song, Choon-Ho; Jang, Kyung-Jeon; Ahn, Chang-Beohm; Kim, Cheol-Hong
  Abstract Objective The purpose of this study is to investigate the difference of treatment effect between Oriental Medicine therapy and Oriental and Western Medicine combination therapy on traffic accident patients. Methods Sixty one traffic accident patients were randomly assigned to the Oriental Medicine therapy group(group I)and Oriental and Western Medicine combination therapy group(group II). Evaluations were made before treatment, after one week treatment and after two weeks treatment using Visual Analog Scale(VAS), Oswestry disability Index(ODI), Neck Disability Index(NDI), Roland Morris Disability Scale(RMDS). The obtained data were analyzed and compared. Results The group I showed significant improvement(p<0.05) according to the VAS, NDI. But that showed insignificant improvement according to the ODI, RMDS. The group II showed significant improvement(p<0.05) according to the VAS, NDI. But that showed insignificant improvement according to the ODI, RMDS. And the difference between the two groups were insignificant according to VAS, NDI, ODI and RMDS. Conclusion There is no significant difference between the two groups after each therapy on traffic accident patients. Further studies are needed for the comparison of the Oriental Medicine therapy and Western Medicine combination therapy.
12 Title
Keywords New Patients; Acupuncture; Moxibustion
Author(s) Kim, Su-Min; Jung, Kyoung-Keun; Lee, Kyoung-Hee; Kwon, Mi-Jung; Jang, Kyung-Jun
  Abstract Purpose In order to profits to investigator who want to know acupuncture and moxbustion treatments are available to what symptoms today and how goes on the clinical lecture for oriental medical students, this research was carried out. Methods The following conclusion were derived from 745 of patients who treated in acupunture & moxibustion department of Dongeui Oriental Medical hospital from the July in 2006 till January in 2007. Results 1. There were 203(27.25%) persons of Lumbago, 101(13.56%) persons of Bel's palsy, 94(12.62%) persons of Omalgia, 80(10.74%) persons of paralysis of limb, 74(9.94%) persons of disordinary sense of limb and etc. 2. There were 395 females(53.02%) and 350 males(46.98%). 3. The patients over 60th were 26.72%, the 50th were 23.08%, the 40th were 22.14%, the 30th were 15.44%, 20th were 8.45%. So the older the more patients. Conclusion In accordance with the above results, acupuncture & moxibustion teatments are good to use degenerative musculoskeletal disease, pain, paralysis and etc.
13 Title
Keywords Essential Blepharospasm; skin temperature; Thermography
Author(s) Gang, Eun-Gyo; Seo, Hyeong-Sik
  Abstract Objectives Benign essential blepharospasm is a progressive neurological disorder characterized by involuntary muscle contractions and spasms of the eyelid muscles. The aim of this study is to provide evidence of differences between facial skin temperaure of the paralyzed side and normal side in Essential Blepharospasm patients. Methods The author studied 13 patients with Essential Blepharospasm. We measured skin temperature of the forehead, zygoma area of the paralyzed side and those of the normal side with The Digital Infrared thermal image. Results There were no significant facial skin temperature differences between the paralyzed side and normal side. Conclusions Thermography is a useful diagnostic tool. But we expect that it is essential to diagnose a disease correctly at an early stage and to find a prompt treatment by introducing and utilizing a simple nonivading diagnofic method other than Thermography.
14 Title
Keywords Hand Paresthesia; Myofascial Pain Syndrome Theory; Pharmacopuncture
Author(s) Oh, Sung-Won; Jeong, Jong-Jin; Kim, Soo-Yeon; Han, In-Sun; Kang, Hyun-Min; Kwon, Ki-Rok; Kim, Byoung-Woo;
  Abstract Objectives Hand paresthesia is common syndrome and the cause is more unknown than known reason. The Purpose of this study were investigated the effects of Myofacial Pain Syndrome theory to make diagnosis and treatment by Pharmacopuncture for the patients of hand paresthesia. Method This study was carried out to established the clinical criteria of hand parethesia. The patients who had past history of diabeics, neuropathy induced by alcohol or drug were excluded, and 86 patients who had hand paresthesia related with unknown-reason was selected by the interview process. And the effects of Pharmacopuncture theory were analyzed using VAS score before and after treatment. Results and conclusions 56.9% of unknown-reason patients are positive at diagnosis by MPS theory. While positive group decrease from to , negative group decrease from to by VAS scores. Positive group was accordingly more effective than negative group. So diagnosis and treatment for hand numbness patients by MPS theory was useful in clinical.
15 Title
Keywords Bee Venom; Pharmacopuncture; Molluscum Contagiosum
Author(s) Park, Sa-Han; Lee, Jong-Young; Kwon, Ki-Rok
  Abstract Objectives This study observed the efficacy of bee venom pharmacopuncture on the treatment of Molluscum Contagiosum commonly manifesting in children. Methods 104 patients admitted for Molluscum Contagiosum at Love Blossoming Oriental medicine clinic from August 2005 to August 2006 were administered with Bee Venom Pharmacopuncture and measured and analyzed changes in symptoms. Results 1. Regardless of age or duration of Molluscum Contagiosum, all 104 patients showed improvement. 2. Recurrence of Molluscum Contagiosum was not noticeable when treated with Bee Venom Pharmacopuncture, and the duration of treatment was significantly shorter than treating with conventional allopathic ointment. 3. Regardless of size or location of Molluscum Contagiosum, most cases improved with one or two treatments. Conclusion Based on above findings, we can deduce Bee Venom Pharmacopuncture has superior anti-viral effects on the pox virus of molluscum contagiosum (MCV1,2).
16 Title
Keywords Side-effect; pharmacopuncture; lubricants; CF and JsD
Author(s) Lee, Jong-Young; Kang, Hyun-Min; Lim, Chung-San; Kwon, Ki-Rok
  Abstract Objectives This study aims to report inflammatory reactions of Pharmacopuncture lubricants (CF and JsD) when administered on GB21, GB20, and TE23 in clinical trials. Methods Follow-up studies were made on each patient's clinical observation and specific managemen. Results 1. Inflammation induced by pharmacopuncture lubricants were limited to local area of administration. 2. Intensity of inflammation was depended on the patient's condition. 3. Inflammation caused pain, skin flare, and discomfort on the patient. Inflammation subsided when dissipating technique was rendered with the conventional acupuncture needle. Conclusions It appears that prolonged usage of pharmacopuncture lubricant on the specific point can induce inflammation, thus using alternative pharmacopuncture is recommended.
17 Title
Keywords Squamous cell lung cancer; Cultured Wild Ginseng Pharmacopuncture; Stable Disease; Disease Free Survival
Author(s) Park, Bong-Ky; Cho, Chong-Kwan; Kwon, Ki-Rok; Yoo, Hwa-Seung
  Abstract Objective To derive further studies evaluating the effectiveness of Cultured Wild Ginseng Pharmacopuncture (CWGP) Therapy on squamous cell carcinoma as a first line. Methods Three cycles (4 weeks/cycle) of CWGP were administered as a dosage of 10 ml per day. Patient was diagnosed with stage IIIB squamous cell carcinoma and refused all therapy of conventional medicine because of old age and cardiac invasion of tumor. Intensive treatment of CWGP for 3 cycles was done on the patient. Computed Topography (CT) was performed to evaluate the therapeutic efficacy. Results After the intravenous infusion of 2 cycles of CWGP, chest CT revealed the mass size and pleural invasion sustained stable disease. After the point injection of 1 cycle of CWGP, chest CT revealed progressive disease. The disease free survival rate was 1 month. Conclusion This case may provide us the possibility that CWGP offers potential benefits for patients with squamous cell lung carcinoma. But this is a single case study and further case-series research should be compensated.
18 Title
Keywords Method of Regulating Ascending Kidney Water and Descending Heart Fire; pharmacopucture; heat symptom in the upper body and coldness in the lower body
Author(s) Kwon, Ki-Rok
  Abstract Objectives The purpose of this study is aimed at diagnosing and suggesting treatment plans for commonly seen clinical manifestation of heat symptom in the upper body and coldness in the lower body, also known as hot above, cold below syndrome. Methods Various reasons attribute to the presence of hot above, cold below syndrome, but mainly contributed by blockage of normal Qi flow by abnormality of heart-kidney root, spleen-stomach axis, and liver-lung axis. Diagnosing these abnormalities and timely alleviation to the healthy state is presented in the study. Results 1For heat in the upper body, Huang Lian Jie Du Tang(黃連解毒湯), CF, or JsD pharmacopuctures are injected on GB21, GB20. Qi stagnation in the thoracic area is treated with BUM injection on CV17. For impairment of transportation and transformation in the middle energizer, BUM pharmacopuncture is injected on CV12. Coldness in the lower energizer was relieved by bee venom or Sweet BV(Bee Venom free from enzymes) on CV6. Conclusion Above proposed methods of regulating water-fire were effective in treating hot above, cold below syndrome in clinical manifestations. But once the symptom subsides, treatment focused on eliminating innate cause should be rendered to achieve more successful results.