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  • [Archive]
  • Journal of Pharmacopuncture
  • pISSN : 2093-6966
  • eISSN : 2234-6856
  • DB Construction : 59 Issues, 667 Articles
Year
Issues
Articles
1 Title
Keywords mountain ginseng Pharmacopuncture(MGP); lung carcinoma; NCI-H460 human non-small lung cancer cells
Author(s) Kwon, Ki-Rok
  Abstract Objectives : This study was performed to examine the anticancer effect of mountain ginseng Pharmacopuncture(MGP) to the nude mouse of lung carcinoma induced by NCI-H460 human nonsmall lung cancer cells. Methods : Human lung cancer (NCI-H460) cells were cultured and applied to evaluate anti-tumor activity in nude mice. After confirmed tumor growth in mice, MGP was treated per 0.1ml/kg dose to intraperitoneal and intravenous injection everyday for four weeks. And checked the changes in body weights, tumor volume, mean survival time and percent, increase in life span, histo-pathological findings, organ weights, and blood chemistry levels. Results : The results of in vivo study showed that MGP may have potential as growth inhibitor of solid tumor induced NCI-H460 without marked side effects. MGP inhibited dosage-dependently the growth of NCI-H460 cell-transplanted solid tumor compared with the control group. And mean survival time of MGP treated group was prolonged comparing with control group. Generally the group of intravenous injection is more effective than intraperitoneal injection. Conclusion : These results were suggested that MGP may be a useful anticancer agent for therapy of human lung cancer. And follow study need for the certain evidence.
2 Title
Keywords NF-xΒ; Akt; COX-2; MMP-9; p53; Bcl-2; Bax; caspase-3; -9; calpain; androgen receptor; xenograft ; 봉독; 전립선암; 세포자멸사
Author(s) Yang, Chang-Yeol; Song, Ho-Sueb
  Abstract
3 Title
Keywords Ginseng; cultivated wild ginseng; antimetastasis activities; AXIN2 gene
Author(s) Jang, S.B.; Lim, C.S.; Jang, J.H.; Kwon, K.R
  Abstract Objective : Ginseng is one of most widely used herbal medicine. Ginseng showed anti-metastasis activities. However, its molecular mechanisms of action are unknown. So we want to report the wild ginseng repress which plays key roles in neoplastic epithelial-mesenchymal transition process. Methods : Treatment of the human colorectal carcinoma LOVO cells and human gastric carcinoma SNU601 cells with the increased concentrations of cultivated wild ginseng extracts resulted in a gradual decrease in the AXIN2 gene expression. Results : Metastasis-suppressor genes, maspin and nm23 was not affected by the treatment of ginseng extracts in LOVO cells. Moreover, the mountain cultivated wild ginseng or mountain wild ginseng are similar in their inhibitory effects on the expression of AXIN2 gene, but are substantially stronger than cultivated ginseng. Conclusion : We described the novel mechanism of wild ginseng-induced anti-metastasis activity by repressing the expression of AXIN2 gene that plays key roles in epithelial-mesenchymal transition process.
4 Title
Keywords Fel Ursi; Pharmacopuncture solution; Keratitis
Author(s) Yoon, Seong-Sik; Seo, Hyung-Sik
  Abstract Objectives : This experimental study was performed to investigate the effectiveness of Fel Ursi Pharmacopunture solution(FUPS) manufactured by using alcohol/water extraction method. To identify the use of it as eyedrops, antibacterial test on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, and Candida albicans was performed. Methods : After treatment FUPS on bacterial species(Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis, we investigated anti-bacterial effects of FUPS on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans in terms of measuring MIC and size of inhibition zone respectively. Results : After FUPS was treated, significant changes of MIC on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans were not observed at all. Conclusions : The present study suggests that FUPS doesn't have anti-bacterial effects on Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum and Candida albicans which cause Keratitis. Perhap These results recommend that FUPS doesn't have anti-bacterial effects but have other mechanism which suppress inflammation.
5 Title
Keywords atopic dermatitis; NC/Nga Mice; Lavender; Lemon; Ucalyptus; IgE; IgG1
Author(s) Kim, Hyeon-Ah; Yun, Mi-Young; Song, Hyang-Hee; Cheong, Kwang-Jo; Yoo, Hwa-Seung
  Abstract Purpose : To investigate the effects of the lavender, lemon and eucalyptus oil mixture on the atopy dermatitis skin lesions induced on NC/Nga Mice by dinitrochlorobenzene (DNCB). Material and Method : For this purpose, we fabricated the oil mixture blending three essential oils (lavender, lemon, eucalyptus : ELL) with one carrier oil (jojoba) and apply it on the atopic dermatitis skin lesions of NC/Nga Mice. Atopic dermatitis in NC/Nga Mice was induced by DNCB treatment on the dorsal skin of mice for 8 weeks. The mixture of ratio of each essential oil drop was 1 (eucalyptus) : 2 (lemon) : 2 (lavender) and this mixture was blended with jojoba oil 50ml (0.025%). The ELL-ointment was supplied for 8 weeks. We evaluated the effects of ELL on cell viability of mouse lung fibroblast, clinical skin features and severity, the level of serum Immunoglobulin (Ig) E & Ig G1, Interleukin (IL)-4, IL-13 and Interferon (IFN)-. Results : ELL showed safety on the cell viability of mouse lung fibroblast compared with control group. The cell viability was measured by SRB method. The effects of ELL on clinical skin features and severity in DNCB-induced dermatitis model of NC/Nga mice was significant compared with control group. EEL also showed significant effects on clinical symptom score compared with control group. Serum IgE & IgG1 level and development of atopy dermatitis skin lesions were evaluated. Serum IgE & IgG1 production was significantly down-regulated in EEL group compared with control group. ELL also down-regulated the levels of IL-4 and IL-13, and up-regulated the level of IFN- compared with control group significantly. Conclusion : ELL was effective on atopy dermatitis by modulating Th2 related factors.
6 Title
Keywords ginsenoside; cultivated wild ginseng; wild ginseng; cultivated ginseng; HPLC
Author(s) Jeong, H.S.; Lim, C.S.; Cha, B.C.; Choi, S.H.; Kwon, K.R.
  Abstract Objectives: The aim of this experiment is to provide an objective differentiation of cultivated ginseng, cultivated wild ginseng, and wild ginseng through component analysis, and to know the change of ginsenoside components in the process for making red ginseng. Methods: Comparative analysis of ginsenoside , Rc, Rd, Re, Rf, and from the cultivated ginseng 4 and 6 years, cultivated wild ginseng, and wild ginseng were conducted using High Performance Liquid Chromatography(hereafter HPLC). And the same analyses were conducted in the process of red ginseng. Results: 1. For content comparison of ginsenoside , Rc, Rd, Rf, and , wild ginseng showed high content, followed cultivated ginseng 4 and 6 years, cultivated wild ginseng showed low content than any other samples. 2. For content comparison of ginsenoside and Re, cultivated ginseng 4 years showed high content, followed wild ginseng and cultivated ginseng 6 years, cultivated wild ginseng showed low content than any other samples. 3. For content comparison of ginsenoside , wild ginseng and cultivated wild ginseng were only showed low content. 4. For content comparison of ginsenoside , cultivated wild ginseng was only showed low content. 5. In the process of red ginseng, ginsenoside , Rc, Rd, and were increased, and ginsenoside Re and were decreased in cultivated wild ginseng. 6. In the process of red ginseng, ginsenoside and were increased, and ginsenoside , Rc, and Re were decreased in cultivated ginseng 4 years. 7. In the process of red ginseng, ginsenoside , Rf and were increased, and ginsenoside Rc and Rd were decreased in cultivated ginseng 6 years. Conclusions: Distribution of ginsenoside contents to the cultivated ginseng, cultivated wild ginseng, and wild ginseng was similar and was not showed special characteristics between samples. And the change of ginsenoside to the process of red ginseng, cultivated ginseng and cultivated wild ginseng were showed different aspect.
7 Title
Keywords Sopungdojeok-tang; Samulsopung-san; Antiinflammation; anti-oxidation; NO; DPPH
Author(s) Seo, Hyung-Sik
  Abstract Objective: This experimental study was performed to investigate the anti-inflammation and anti-oxidant effects of Sopungdojeok-tang(SPDJT) and Samulsopung-san(SMSPS) which were used to treat patient with atopic dermatitis Methods: Anti-inflammation and anti-oxidant effects of SPDJT and SMSPS were measured by the inhibitory ability of Nitric oxide(NO) production and the scavenging for 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical. Results: 1. SPDJT and SMSPS were not showed cell toxicity. 2. In inhibitory effects against NO production, all groups of SPDJT were showed anti-inflammation, but all groups of SMSPS were not showed anti-inflammation. There were statistical significances between and 4, in SPDJT groups. 3. In DPPH radical scavenging activity, all groups of SPDJT and all groups of SMSPS were showed anti-oxidant effects. There were statistical significances between SPDJT group and 4, SMSPS groups. Conclusion: These results suggest that the SPDJT groups are better than the SMSPS groups in antiinflammation and anti-oxidant effects.
8 Title
Keywords Side-effect; pharmacopuncture; lubricants; CF and JsD
Author(s) Kim, B.W.; Kwon, K.R.
  Abstract Objectives:This study was performed to examine the side effect of inflammatory reactions of Pharmacopuncture lubricants (CF and JsD) using animal model. Methods:Pharmacopuncture lubricants (CF and JsD) were treated repeatedly the same point in Sprague-Dawley rats. And after finish the treatment, we obtain the tissue of muscle layer in rats where Pharmacopuncture lubricants were treated. And we observed the tissue how the change occurred in HE staining. Results: 1. There was no evidence to make inflammation induced by pharmacopuncture lubricants except 4 weeks treated group. 2. Only 4 weeks treated group(CF, JsD) were showed some infectional tissue's view in H-E staining. But it is not certain due to Pharmacopuncture lubricants. Conclusion:It appears that prolonged usage of pharmacopuncture lubricant on the specific point can induce inflammation, so we may be careful for treating time and point using pharmacopuncture lubricant in clinics.
9 Title
Keywords Male legs; measurement; cun (寸); Measurement of the Bone in Neijing Lingshu
Author(s) Yu, Shin-Jong; Kwon, Oh-Sang; Ahn, Seong-Hoon; Kim, Young-Jin; Kim, Kyung-Sik; Sohn, In-Chul; Kim, Jae-Hyo
  Abstract Objective : Standard of measurement in Korean Medicine has been changed in dynasty and location. Thus, cun (寸) and chi (尺) as unit of measurement for meridians and acupoints could be recognized as the length of equally divided portions of a certain long bone or the distance between two anatomical landmarks and as an symbolical meaning to date. The goal of this study is to propose a new standard measurement in the metric system for the relative measurement of cun and chi as unit of measurement for meridians and acupoints in normal male legs. Methods : This study was conducted by gauging each parts of normal male legs in the metric system and comparing to the relative measurement of cun and chi as follows; to calculate 1 cun, the length of each parts was divided into the unit of cun referred to Measurement of the Bone in Neijing Lingshu (靈樞 骨度篇); it was compared the unit of cun referred to Measurement of the Bone in Neijing Lingshu with cun which was calculated by dividing subject's height into 75 cun, respectively. Result : There has no significant difference in length of 1 cun among each leg's areas based on a standard of subject's height. The unit of cun by the metric length in the legs was similar to the unit of cun referred to Measurement of the Bone in Neijing Lingshu based on each subject's height. Conclusion : It is suggested that an unit of cun as the measurement for meridians and acupoints in the male legs should be considered to the ranges from 2.4cm to 2.6cm.
10 Title
Keywords Leech therapy; Hirudo medicinalis; Medicinal leech; Hirudotherapy
Author(s) Jang, Hyo-Kil; Heo, Dong-Seok
  Abstract Objective : To review the trend of the study related to Leech therapy and to establish the hereafter direction for the study on Leech therapy. Methods : I reviewed and analyzed all the theses published in Domestic and Foreign research institution from 1990 to 2009. Results : The following results were obtained in this study. 1. Analyzed number of theses published, there was no significance per year. 2. Classified by theme of journal, journals related to surgery were most(41 journals, 75.92%) among 54 kinds. 3. Classified by theses by research method and thesis types, case report accounted for nearly twothirds (52 pieces, 68.42%) of all theses and consideration of document was next(9 pieces, 11.84%). 4. With the most case of venous congestion after plastic and reconstructive surgery(33 pieces, 63.46%), leech therapy was effective on illnesses such as haematoma, macroglossia, purpura, varicous vein, avulsion injury, neurovascular compression, diabetic neuropathy, penoscrotal oedema, buerger's disease, rheumatoid arthritis. 5. Two most appeared adverse effects were anemia and infection. Immediate blood transfusion was done for recovering anemia and prophylactic 3rd generation antibiotics to infection were emphasized in more than half of case reports. 6. All of consideration of documents was retrospective study of cases related leech therapy and 3 pieces of them emphasized prophyratic antibiotic treatment for preventing infection. 7. The study of clinical trail type started first in 2002 and osteoarthritis of knee and carpometacarpal joint were main target. As see above result, Leech therapy was effective cure and could be used in disease induced by venous congestion. And I think that it is necessary to perform additional study related to solution of problems about leech therapy and protocol for using in clinical practice.
11 Title
Keywords Amyotrophic lateral sclerosis(ALS); ALS Functional Rating Scale-Revised(ALSFRS-R); Medical Research Council(MRC) Scale; SAAM-acupuncture; Pharmacopuncture therapy
Author(s) Kim, Hyun-Sik; Song, Bong-Kuen; Park, Man-Yong; Lim, Na-Ra; Kim, Sung-Ha; Kim, Sung-Chul
  Abstract Objectives : This study was to investigate the effects of Oriental medical treatment on ALS. Methods : We investigated 12 ALS patients which were admitted to Gwang-Ju O.M.hospital for 3 months and follow-up at 8 months after discharge. All patients were treated by SAAM-acupuncture, herb medication, Bee venom Pharmacopuncture therapy, Needle-embedding therapy, etc and after discharge self-therapy at home. We evaluated patients using the Amyotrophic lateral sclerosis Functional Rating Scale-Revised(ALSFRS-R), Medical Research Council (MRC) Scale. Results : After 1 month, mean ALSFRS-R score of patients was , 2 months , 3 months , 1 year and mean MRC Scale of patients was , 2 months , 3months . But in both cases, the variation was not statistically significant. Conclusions : We think that the results of this case be a pilot study that proves the effect of Oriental Medical treatment on ALS.
12 Title
Keywords traumatic lumbar burst fracture; spinal cord injury; mae-sun therapy; TUG; SCIM II; CIC
Author(s) Kwon, Gi-Sun; Park, Jung-Ah; Noh, Ju-Hwan; Kim, Cheol-Hong
  Abstract Objective : Traumatic lumbar burst fracture causes significant spinal cord injury. This report is intended to estimate the efficacy using oriental treatment on a patient with lumbar burst fracture and spinal cord injury. Methods : From 21th December, 2009 to 5th February, 2010, 1 female inpatient diagnosed with lumbar burst fracture and spinal cord injury was treated with general oriental medicine therapy : mae-sun therapy ; acupuncture ; moxibustion ; pharmacopuncture ; physical therapy and herbal medication. TUG, SCIMII and VAS were used for evaluation of gait disturbance and pain in both feet. Measurement of self voiding amount and remaining amount through CIC was used for evaluation of neurogenic vesical dysfunction. Results : The patient showed a certain degree of improvement in gait disturbance, pain in both feet and neurogenic vesical dysfunction through above evaluation methods. Conclusion : Oriental treatments such as mae-sun therapy, acupuncture and moxibustion therapy, pharmacopuncture therapy and herbal medication can be effective for spinal cord injury due to traumatic lumbar burst fracture.